Reprogramming Escherichia coli pyruvate-forming reaction towards chorismate derivatives production

Microbial metabolic pathway engineering is a potent strategy used worldwide to produce aromatic compounds. We drastically rewired the primary of Escherichia coli aromatics and their derivatives. The E. was compartmentalized into production energy modules. focused on pyruvate-forming reaction in biosynthesis some compounds as connecting those strains were engineered show no growth unless pyruvate synthesized along with interest production. Production salicylate maleate demonstrated confirm our strategy's versatility. In production, yield against theoretical yield, rate reached 12.0 g L?1, 67%, up fourfold compared that previous reports, respectively; these are highest values microbes knowledge. results reveal strongly promotes